Download PDF by Andrei G. Pakhomov, Damijan Miklavcic, Marko S. Markov: Advanced electroporation techniques in biology and medicine

By Andrei G. Pakhomov, Damijan Miklavcic, Marko S. Markov

ISBN-10: 1439819068

ISBN-13: 9781439819067

"A mirrored image of the serious research of the results of electromagnetic fields on residing tissues that has taken position over the last a number of many years, this booklet discusses the theoretical and experimental facts and issues the consequences of sturdy electromagnetic fields and/or electrical pulses and their significance in drugs and biology. The authors current the elemental options utilized in electroporation and the complex equipment for construction of nanopores, highlighting their simple technology and scientific purposes. subject matters comprise nano electroporation, vintage electroporaiton, experimental proof for electroporation of dwelling cells, and electroporation for melanoma and wound healing"--Provided by way of writer. learn more... content material: fundamentals of Electroporation actual Chemical idea of Membrane Electroporation and Electrotransfer of Biogenic brokers, E. Neumann and S. Kakorin Bioelectric influence of extreme Nanosecond Pulses, K.H. Schoenbach caused Transmembrane Voltage-Theory, Modeling, and Experiments, T. Kotnik and G. Pucihar Electroporation: A assessment of easy difficulties in idea and scan, M.S. Markov Mechanisms of Electroporation in Lipid structures Electrodeformation, Electroporation, and Electrofusion of Cell-Sized Lipid Vesicles, R. Dimova Fluorescent equipment in overview of Nanopore Conductivity and Their Computational Validation, M. Kotulska, W. Dyrka, and P. Sadowski Electroporation of Lipid Membranes: Insights from Molecular Dynamics Simulations, M. Tarek and L. Delemotte Nanoscale Restructuring of Lipid Bilayers in Nanosecond electrical Fields, P.T. Vernier Mechanisms of Electroporation of Cells Nanopores: a unique Transmembrane Passageway in Electroporated Cells, A.G. Pakhomov and O.N. Pakhomova version of mobile Membrane Electroporation and Transmembrane Molecular delivery, D. Miklavcic and L. Towhidi Kinetics of Pore Formation and Disappearance within the telephone in the course of Electroporation, G. Saulis the heartbeat Intensity-Duration Dependency for mobilephone Membrane Electroporation, D. Miklavcic, G. Pucihar, A.M. Lebar, J. Krmelj, and L. Towhidi Mechanisms of Electroporation in Tissues Drug-Free, reliable Tumor Ablation by way of Electroporating Pulses: Mechanisms That Couple to Necrotic and Apoptotic mobilephone loss of life Pathways, A.T. Esser, K.C. Smith, T.R. Gowrishankar, and J.C. Weaver Gene Electrotransfer: From uncomplicated methods to Preclinical functions, J.-M. Escoffre, A. Paganin-Gioanni, E. Bellard, M. Golzio, M.-P. Rols, and J. Teissie Technical issues Modeling electrical box Distribution In Vivo, N. Pavselj, A. Zupanic, and D. Miklavcic suggestions of Electroporation Pulse new release and evaluate of electrical Pulse turbines for phone and Tissue Electroporation, M. Rebersek and D. Miklavcic iteration of Ultrashort Pulses, J.F. Kolb Nanosecond Pulsed electrical box supply to organic Samples: problems and strength options, A. Silve, J. Villemejane, V. Joubert, A. Ivorra, and L.M. Mir purposes of Electroporation Translation of Electroporation-Mediated DNA supply to the sanatorium, L.C. Heller and R. Heller medical Electrochemotherapy: The Italian event, C.R. Rossi and L.G. Campana Tumor Blood Flow-Modifying results of Electroporation and Electrochemotherapy-Experimental facts and Implications for the remedy, T. Jarm, M. Cemazar, and G. Sersa lectrochemotherapy as a part of an Immunotherapy process within the therapy of melanoma, J. Gehl ombined electric box and Ultrasound: A Nondrug-Based approach for Tumor Ablation, P.F. Forde, C. Twomey, G.C. O' Sullivan, and D. M. Soden mixed Modality treatment: Electrochemotherapy with Tumor Irradiation, G. Sersa, S. Kranjc, and M. Cemazar Irreversible Electroporation in drugs, B. Rubinsky nutrition and Biomaterials Processing Assisted through Electroporation, N. Lebovka and E. Vorobiev In Vivo Electroporation: a massive harm Mechanism in electric surprise Trauma, I. Barakat, J. Gallaher, H. Chen, and R.C. Lee Index summary: Reflecting the serious research of the results of electromagnetic fields on dwelling tissues that has taken position through the years, this name summarizes the experimental findings and theories regarding permeabilization of biomembranes through pulsed electrical fields. it truly is meant extensively for biomedical and actual scientists, engineers, and clinicians. learn more...

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H. , Wideband, high-amplitude, pulsed antennas for medical therapies and medical imaging, in Proceedings 2009 International Conference on Electromagnetics in Advanced Applications, September 14–18, 2009, Torino, Italy, 2009b, pp. 580–583, IEEE catalog number: CFP0968B-CDR. , 2009). , 2008) with a coaxial exposure chamber as the load. The lethality of such wideband pulses was studied by measuring the trypan blue uptake in pancreatic cancer cells (Panc-1). With 1000 pulses with a duration of 180 ps and electric fields of 150 kV/cm applied to Panc-1 cells in cell culture media, trypan blue uptake of 10%–15% was measured, almost independent of temperature, in the range from 23°C to 35°C.

The spatial resolution of an electric field generated in tissue depends on the pulse duration and the permittivity of the tissue. , 2009b). Imaging is based on the measurement of changes in the complex permittivity of tissue and may complement methods based on the measurement of other physical parameters, such as X-ray computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound. , 2002) and can therefore be easily differentiated from normal tissue. In such an imaging system, the electromagnetic waves are focused on a target inside the body.

The two processes are coupled. , 2008). , 2008). , 2001). , PDGF and transforming growth factor beta [TGF-β]). The release of this growth factor is essential for wound healing. In addition to growth factor release, recent studies have also shown that nsPEFs have an antibacterial effect. The concentration of Staphylococcus aureus decreased considerably after treatment with nsPEF-activated platelet gel and decreased significantly compared with bacteria treated with thrombin. 2 Treating Skin Cancer A wide range of stimuli with either plasma membrane or intracellular membrane targets can be responsible for the induction of apoptosis.

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Advanced electroporation techniques in biology and medicine by Andrei G. Pakhomov, Damijan Miklavcic, Marko S. Markov


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